doi: 10.5732/cjc.010.10599

Overexpression of centromere protein H is significantly associated with breast cancer progression and overall patient survival

Wen-Ting Liao, Yan Feng, Men-Lin Li, Guang-Lin Liu, Man-Zhi Li, Mu-Sheng Zeng, Li-Bing Song

State Key Laboratory of Oncology in South China; Department of Experimental Research, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, P. R. China. LB.Song1@gmail.com

[Abstract] Breast cancer is one of the leading causes of cancer death worldwide. This study aimed to analyze the expression of centromere protein H (CENP-H) in breast cancer and to correlate it with clinicopathologic data, including patient survival. Using reverse transcription-polymerase chain reaction and Western blotting to detect the expression of CENP-H in normal mammary epithelial cells, immortalized mammary epithelial cell lines, and breast cancer cell lines, we observed that the mRNA and protein levels of CENP-H were higher in breast cancer cell lines and in immortalized mammary epithelial cells than in normal mammary epithelial cells. We next examined CENP-H expression in 307 paraffin-embedded archived samples of clinicopathologically characterized breast cancer using immunohistochemistry, and detected high CENP-H expression in 134 (43.6%) samples. Statistical analysis showed that CENP-H expression was related with clinical stage (P = 0.001), T classification (P = 0.032), N classification (P = 0.018), and Ki-67 (P < 0.001). Patients with high CENP-H expression had short overall survival. Multivariate analysis showed that CENP-H expression was an independent prognostic indicator for patient survival. Our results suggest that CENP-H protein is a valuable marker of breast cancer progression and prognosis.

Chinese Journal of Cancer 2011, Volume: 30, Issue 9, Page: 627-637

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Wen-Ting Liao, Yan Feng, Men-Lin Li, Guang-Lin Liu, Man-Zhi Li, Mu-Sheng Zeng, Li-Bing Song. Overexpression of centromere protein H is significantly associated with breast cancer progression and overall patient survival. Chin J Cancer. 2011, 30(9):627-637. doi:10.5732/cjc.010.10599


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